DNA
Part:BBa_K1641206:Design
Designed by: Jianheng Liu Group: iGEM15_SYSU_CHINA (2015-09-14)
FRT(reverse)-loxP(forward)-pBAD(reverse)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 147
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence has two primer sites FRT and loxP for further testing in our experiment. Two primers are: GCACCTTGACTCTGACAATCCT (forward) GCCTGTGCTAATGGTGATGACT (reverse) they both have a high annealing temperature (about 58C). They can be used as qPCR primers.