DNA

Part:BBa_K1641206:Design

Designed by: Jianheng Liu   Group: iGEM15_SYSU_CHINA   (2015-09-14)


FRT(reverse)-loxP(forward)-pBAD(reverse)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has two primer sites FRT and loxP for further testing in our experiment. Two primers are: GCACCTTGACTCTGACAATCCT (forward) GCCTGTGCTAATGGTGATGACT (reverse) they both have a high annealing temperature (about 58C). They can be used as qPCR primers.


Source

References